An in-Vitro Examination of Human Hair Keratin Nanofibrous Matrices as an Alternative Biomaterial for Tissue Regeneration
2020-08-10 17:38:05
报告题目：An in-Vitro Examination of Human Hair Keratin Nanofibrous Matrices as an Alternative Biomaterial for Tissue Regeneration
报告时间：2020年8月12日13:30
报告地点：高新大厦15楼会议室1
报告人：苏婉婷（李花琼组）
摘要：Natural polymers such as proteins and polysaccharides are potential materials as templates for tissue regeneration due to their natural bio-inductive abilities to improve cell-material interactions. However, most of these materials still present some shortcomings due to their animal origins. From this perspective, hair keratin emerges as an attractive material of human origin to be used as templates for tissue regeneration because it can be extracted from the unlimited supply of human hair. In this work, we extracted keratin from human hair, fabricated it into nanofibrous matrices via a scalable technique, electrospinning and evaluated their potential as fibrous templates for supporting cell growth. We cultured primary human dermal fibroblasts (HDFs) on these matrices and evaluated their cell compliance in terms of cell distribution, cytoskeleton development, proliferation and the role of keratin matrices in extracellular matrix (ECM) production. Our results showed that keratin fibrous matrices with beadless morphology were electrospun with a mean fibre diameter of 0.70 ± 0.09 µm for random fibres and 0.74 ± 0.10 µm for aligned fibres. Results from cell studies showed that proliferation and metabolic activity of HDFs on keratin matrices were comparable to the positive control and significantly higher on aligned keratin fibres compared to random keratin fibres on day 3 and day 5 after cell seeding. Live/dead staining indicated that HDFs cultured on the keratin matrices could form cellular networks according to the architecture of the matrices Besides, F-actin staining revealed that the HDFs exhibited their typical spindle morphology and responded well to the topography of the keratin matrices. We have also shown that keratin matrices could stimulate the HDFs to secrete and deposit ECM proteins, namely fibronectin and collagen. In this work, we showed that keratin fibrous matrices provide a conducive environment to facilitate the growth of primary HDFs and promote ECM production. From these results, we concluded that electrospun human hair keratin matrices have the potential to be developed into templates for tissue regeneration.
报告时间：2020年8月12日13:30
报告地点：高新大厦15楼会议室1
报告人：苏婉婷（李花琼组）
摘要：Natural polymers such as proteins and polysaccharides are potential materials as templates for tissue regeneration due to their natural bio-inductive abilities to improve cell-material interactions. However, most of these materials still present some shortcomings due to their animal origins. From this perspective, hair keratin emerges as an attractive material of human origin to be used as templates for tissue regeneration because it can be extracted from the unlimited supply of human hair. In this work, we extracted keratin from human hair, fabricated it into nanofibrous matrices via a scalable technique, electrospinning and evaluated their potential as fibrous templates for supporting cell growth. We cultured primary human dermal fibroblasts (HDFs) on these matrices and evaluated their cell compliance in terms of cell distribution, cytoskeleton development, proliferation and the role of keratin matrices in extracellular matrix (ECM) production. Our results showed that keratin fibrous matrices with beadless morphology were electrospun with a mean fibre diameter of 0.70 ± 0.09 µm for random fibres and 0.74 ± 0.10 µm for aligned fibres. Results from cell studies showed that proliferation and metabolic activity of HDFs on keratin matrices were comparable to the positive control and significantly higher on aligned keratin fibres compared to random keratin fibres on day 3 and day 5 after cell seeding. Live/dead staining indicated that HDFs cultured on the keratin matrices could form cellular networks according to the architecture of the matrices Besides, F-actin staining revealed that the HDFs exhibited their typical spindle morphology and responded well to the topography of the keratin matrices. We have also shown that keratin matrices could stimulate the HDFs to secrete and deposit ECM proteins, namely fibronectin and collagen. In this work, we showed that keratin fibrous matrices provide a conducive environment to facilitate the growth of primary HDFs and promote ECM production. From these results, we concluded that electrospun human hair keratin matrices have the potential to be developed into templates for tissue regeneration.
